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Swarna Latha, L.
- Evaluation of Anticancer and Antibacterial Activity of Shpaeranthus Amaranthoides
Abstract Views :141 |
PDF Views:3
Methods: The elutes of the column chromatography by using ethylacetate and ethanol of Sphaeranthus amaranthoides were tested against multi drug resistant pathogenic bacteria they are E.coli ATCC 25922, Staphylococcus aureus ATCC 25923 and pseudomonal aeroginosa 27853. by using agar well diffusion method and MIC. The cytotoxic effect of the fraction on Hep2 cells were assayed using 3-(4,5-Dimethylthioazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT).
Results: Among the 3 organisms tested the extract showed a greater activity on pseudomonas then on staphylococcu aureus followed by E.coli. in both, agar well diffusion method and MIC. The 1:8 dilution of the ethylacetate fraction was non toxic to normal cells and also had both anticancerous and antiproliferative activities against cancerous cells.
Conclusion: The study confirm the antibacterial and anticancerous activities of Sphaeranthus amaranthoides extract and is hence therefore a potential drug that requires further studies and development.
Authors
Affiliations
1 Department of Biotechnology, Sathyabama University, Chennai-600119, IN
2 Department of Bioorganic Chemical Laboratory, CLRI, Chennai-600020, IN
1 Department of Biotechnology, Sathyabama University, Chennai-600119, IN
2 Department of Bioorganic Chemical Laboratory, CLRI, Chennai-600020, IN
Source
Biometrics and Bioinformatics, Vol 3, No 3 (2011), Pagination: 121-124Abstract
Aim: The aim of the present study is to evaluate the antibacterial and anticancerous activity of Sphaeranthus amaranthoides.Methods: The elutes of the column chromatography by using ethylacetate and ethanol of Sphaeranthus amaranthoides were tested against multi drug resistant pathogenic bacteria they are E.coli ATCC 25922, Staphylococcus aureus ATCC 25923 and pseudomonal aeroginosa 27853. by using agar well diffusion method and MIC. The cytotoxic effect of the fraction on Hep2 cells were assayed using 3-(4,5-Dimethylthioazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT).
Results: Among the 3 organisms tested the extract showed a greater activity on pseudomonas then on staphylococcu aureus followed by E.coli. in both, agar well diffusion method and MIC. The 1:8 dilution of the ethylacetate fraction was non toxic to normal cells and also had both anticancerous and antiproliferative activities against cancerous cells.
Conclusion: The study confirm the antibacterial and anticancerous activities of Sphaeranthus amaranthoides extract and is hence therefore a potential drug that requires further studies and development.
Keywords
Sphearanthus Amaranthoides, Antibacterial Activity, Anticancerous Activiy, HEp2 Cells, MTT Assay.
- Protective Role of Sphaeranthus Amaranthoides Against Antidiarrhoeal, Antibacterial and Antipyretic Activity in Wister Rats-Using of Column Cromotography Extract and Lorke Method
Abstract Views :135 |
PDF Views:3
The present investigation clearly reveals the antibacterial nature of this plant and suggests that this plant could be exploited in the management of diseases caused by these bacteria in human. Further ethanolic extract of SPA in graded doses reduced diarrhoea and thereby lending support to use SPN in folklore medicine against chronic diarrhea. The phytochemical analysis revealed that the active principle, which is a phenolic compound present in the ethanolic extract of SPA is thus responsible for the antibacterial and antidiarroheal activity. So it has become expedient to examine scientifically the protective effects of these herbal plants and thus there has been a continuing search for new and more potent antibiotics (Heisig, 2001). Therefore there is an increased need for research in the investigations on plants as a source of human disease management (Aiyelagabe et al. 2000; Mouniswamy et al. 2002; Woldemichael et al. 2003. In addition, The ethanol extract of sphaeranthus amaranthoides was investigated for antipyretic activity in rats using yeast. In rats, yeast (20 ml/kg body weight, s.c) caused elevation of rectal temperature of 0.50°c 18h after administration.The extract (100 and 200 mg/kg body weight, s.c) produced a significant (p<0.05) dose dependent inhibition of temperature elevation.). It is also observed that the role of mathematical models in biological and clinical research is vital in nature to ensure the accuracy and the effectiveness of the drug developed.
Authors
Affiliations
1 Department of Biotechnology, Sathyabama University, Chennai, IN
2 Department of Bioorganic Chemical Laboratory, CLRI, Chennai, IN
1 Department of Biotechnology, Sathyabama University, Chennai, IN
2 Department of Bioorganic Chemical Laboratory, CLRI, Chennai, IN
Source
Biometrics and Bioinformatics, Vol 3, No 4 (2011), Pagination: 215-220Abstract
Consumption of medicinal herbs is tremendously increasing in order to identify alternative approaches to improve the quality of life and maintain a good health for the past 10 years. Antibiotics provide the main basis for the therapy of bacterial infections. The preliminary phytochemical study, in vitro antibacterial activity and antidiarrhoeal activity of the ethanolic extract of Spheranthus amaranthoides having ethnomedicinal uses were investigated. The preliminary phytochemical analysis of the extracts revealed the presence of flavonoids, carbohydrate, tannins, saponins, steroids, glycosides, terpenoids and alkaloids. The extracts were subjected for screening of in vitro antibacterial activity against selected major disease causing pathogens viz. Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Enterococcus faecalis and Escherichia coli at the concentrations of 50, 100, 200, and 400μg/ml by disc diffusion assay method. The results of antibacterial activity revealed good inhibitory activity against all the tested pathogens. Spheranthus amaranthoides also caused a significant (p<0.001) castor oil induced antidiarrhoeal effect in rats. The results suggest that these activites might be due to the presence of the potent phytochemicals in the extract of Spheranthtus amaranthoides. But despite effort to maintain good health, man and animals alike still confront disease conditions which are due to exposure to physiopathological agent and medicinal plants such as Sphaeranthus amaranthoides can be used as a therapeutic agent.The present investigation clearly reveals the antibacterial nature of this plant and suggests that this plant could be exploited in the management of diseases caused by these bacteria in human. Further ethanolic extract of SPA in graded doses reduced diarrhoea and thereby lending support to use SPN in folklore medicine against chronic diarrhea. The phytochemical analysis revealed that the active principle, which is a phenolic compound present in the ethanolic extract of SPA is thus responsible for the antibacterial and antidiarroheal activity. So it has become expedient to examine scientifically the protective effects of these herbal plants and thus there has been a continuing search for new and more potent antibiotics (Heisig, 2001). Therefore there is an increased need for research in the investigations on plants as a source of human disease management (Aiyelagabe et al. 2000; Mouniswamy et al. 2002; Woldemichael et al. 2003. In addition, The ethanol extract of sphaeranthus amaranthoides was investigated for antipyretic activity in rats using yeast. In rats, yeast (20 ml/kg body weight, s.c) caused elevation of rectal temperature of 0.50°c 18h after administration.The extract (100 and 200 mg/kg body weight, s.c) produced a significant (p<0.05) dose dependent inhibition of temperature elevation.). It is also observed that the role of mathematical models in biological and clinical research is vital in nature to ensure the accuracy and the effectiveness of the drug developed.